lumbar by bheru lal
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LUMBAR PUNCTURE
Indications
1. Suspected CNS infection
2. Suspected subarachnoid hemorrhage
3. Therapeutic reduction of cerebrospinal fluid (CSF) pressure
4. Sampling of CSF for any other reason
Contraindications
1. Local skin infections over proposed puncture site (absolute contraindication)
2. Raised intracranial pressure (ICP); exception is pseudotumor cerebri
3. Suspected spinal cord mass or intracranial mass lesion (based on lateralizing
neurological findings or papilledema)
4. Uncontrolled bleeding diathesis5. Spinal column deformities (may require fluoroscopic assistance)
6. Lack of patient cooperation
Materials
1. Lumbar puncture tray (to include 20 or 22 gauge Quinke needle with stylet, prep
solution, manometer, drapes, tubes, and local anesthetic)
2. Universal precautions materials
Pre-procedure patient education
1. Obtain informed consent
2. Inform patient of possibility of complications (bleeding, persistent headache,
infection) and their treatment
3. Explain the major steps of the procedure, positioning, and postpocedure care
Procedure
1. Assess indications for procedure and obtain informed consent as appropriate2. Provide necessary analgesia and/or sedation as required
3. Position patient: lateral decubitus position with fetal ball curling up, or
seated and leaning over a table top; both these positions will open up the
interspinous spaces (see Figure 1)
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Figure 1: Positioning patient for lumbar puncture
4. Locate landmarks: between spinous processes at L4-5, L3-4, or L2-3 levels
(see Figure 2). On
obese patients, find
the sacral
promontory; the
end of this
structure
marks the L5- S1
interspace. Use
this reference to
locate L4-5 for
the entry
point. You will
aim the needletowards the
navel.
Figure 2. Anatomy of
lumbar spine
5. Prep anddrape the area
after
identifying
landmarks.
Use lidocaine
1% with or
without
epinephrine to
anesthetize the skin
and the deeper tissuesunder the insertion
site
6. Assemble needle and
manometer. Attach
the 3-way stopcock to
manometer
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7. Insert Quinke needle bevel-up through the skin and advance through the
deeper tissues. A slight pop or give is felt when the dura is punctured. Angle
of insertion is on a slightly cephalad angle, between the vertebra (Figure 3). If
you hit bone,
partially withdraw
the needle,
reposition, and re-
advance
Figure 3
8. When CSF flows,
attach the 3-way
stopcock and
manometer.
Measure ICPthis
should be 20 cm orless. Note that the
pressure reading is not reliable if the patient is in the sitting position
9. If CSF does not flow, or you hit bone, withdraw needle partially, recheck
landmarks, and re-advance
10. Once the ICP has been recorded, remove the 3-way stopcock, and begin filling
collection tubes 1-4 with 1-2 ml of CSF each
Tube 1: glucose, protein, protein electrophoresis
Tube 2: Grams stain, bacterial and viral cultures
Tube 3: cell count and differential
Tube 4: reserve tube for any special tests
11. After tap, remove needle, and place a bandage over the puncture site. Instruct
patient to remain lying down for 1-2 hours before getting up
NOTES:
1. Insertion of the needle bevel-up minimizes dural trauma
2. A traumatic bloody tap occurs when a spinal venous plexus is penetrated.
Often the fluid will clear as succeeding tubes are filled. Spin down the first
tube: if red blood cells have been in the spinal fluid for some time (forexample, subarachnoid hemorrhage), xanthochromia will be present in the
supernatant fluid. If the fluid is clear after it is spun down, the tap was only
traumatic
3. In some cases, conscious sedation is helpful in reducing patient anxiety and
allowing maximal spinal flexion
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COMPLICATIONS, PREVENTION, AND MANAGEMENT
Complication Prevention Management
Bleeding from puncture
site post-tap
None
Local pressure
Bloody spinal fluid
None
Withdraw needle andperform tap at interspace
either above or below
Infection
Do not perform tap through
infected skin
Use sterile technique
Antibiotics
Post-tap persisting
headache
Use pencil-tipped needle if
possible; insert needle bevel-up
Post-procedure epidural
blood patch by anesthesia
consultant
Documentation in the medical record
Include in your note a brief history and physical examination of the patient, the
reasons for performing the lumbar puncture, and consent. Note in particular a brief
examination of the cranial nerves, presence or absence of papilledema, or any other
lateralizing neurological finding. Also include a brief note of examination of the patients
spine with attention to any obvious spinal deformity.
Document position of patient during the procedure, opening pressure, and
clarity/color of the CSF. Once results of the CSF analysis are available, they can be
appended to your note.
NORMAL CSF VALUES
Items for evaluation of person learning this procedure
1. Anatomy of lumbar spine
2. Indications of procedure
3. Contraindications for procedure
4. Interaction between physician, staff, patient, and/or family
5. Sterile technique, universal precautions
Parameter Normal Values
Protein 15-45 mg/dl
Glucose 50-80 mg/dl
WBC < 5 mm3
RBC 0-5
Opening pressure 5-20 cm
Clarity, color Clear and colorless
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6. Technical ability
7. Appropriate documentation
8. Understanding of potential complications and their correction
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