BsrG1 ApaI Bgl II EcoRI HindIII KpnI Sal IXhoISmaI

FseI

EcoRV

CTG TAC AGG GCC cga tat cag atc tgg cga att caa gct tgg tac ccg ggt cga ctc gagL Y R A R Y Q I W R I Q A W Y P G R L E

tct ggc cgg CCG CGG GGA TCC AGA CAT GAT AAG ATA CAT TGAS G R P R G S R H D K I H *

BsrG1 ApaI Bgl II EcoRI HindIII KpnI Sal IXhoISmaI

FseI

EcoRV

CTG TAC AGG GCC cag ata tca gat ctg aat tca agc ttg gta ccc ggg tcg act cga gtcL Y R A Q I S D L N S S L V P G S T R V

tgg ccg gCC GCG GGG ATC CAG ACA TGAW P A A G I Q T *

BsrG1 ApaI Bgl II EcoRI HindIII KpnI Sal IXhoISmaI

FseI

EcoRV

CTG TAC AGG GCC cga gat atc aga tct gaa ttc aag ctt ggt acc cgg gtc gac tcg agtL Y R A R D I R S E F K L G T R V D S S

ctg gcc ggC CGC GGG GAT CCA GAC ATG ATA AGA TAC ATT GAT GAG TTT GGA CAA ACC ACAL A G R G D P D M I R Y I D E F G Q T T

ACT AGA ATG CAG TGAT R M Q *

pCMV-sGFPa

pCMV-sGFPb

pCMV-sGFPc

710 BsrGI716 ApaI722 EcoRV728 BglII737 EcoRI743 HindIII749 KpnI753 SmaI758 SalI763 XhoI772 FseI

773 NaeI773 NgoMIV775 EagI778 SacII

897 PsiI928 MfeI1018 AceII1018 NheI

1379 AflIII1379 PciI

ScaI 2750

SspI 3074

BbeI 3494KasI 3494NarI 3494SfoI 3494

BsaAI 3959SnaBI 3959

BspEI 4268PpuMI 4343

StuI 4409

pCMV-sGFPa.str

4473 base pairsUnique Sites

GFPCMV

Promoter