bab i uji sensivitas bakteri

7/23/2019 BAB I Uji Sensivitas Bakteri

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LAPORAN PRAKTIKUM MIKROBIOLOGI

“UJI SENSITIVITAS BAKTERI TERHADAP ANTIBIOTIKA”

DISUSUN OLEH :

1. Dhimas Khalis Nl Ha!im "#$1##1$$%#&#. Diah K'is(a "#$1##1$$%)&

). Di(*a P+'ma,asa'i "#$1##1$$%-&

. Eli/a0+,h a'2li(+ S "#$1##1$$-3&

4AKULTAS 4ARMASI

UNIVERSITAS PANASILA

JAKARTA

#$1)

BAB I



PENDAHULUAN

I.I La,a' B+la!a(5

Pa*a ilm mi!'20i2l25i i(i !i,a m+m6+la7a'i 0a(8a! ,+(,a(5 7asa*97asa* '+(i!

8a(5 *is+0, 75a *+(5a( mi!'20a a,a 6'2,is,a *ima(a a*a(8a ;i'i9;i'i(8a

!+!+'a0a,a((8a a(,a'a s+sama(8a s+6+',i 75a *+(5a( !+l2m62! 2'5a(ism+ lai((8a

6+(55(aa( *a( 6+'a(a((8a *alam !+s+ha,a( s+',a !+s+7ah,+'aa( !i,a.

Mi!'22'5a(ism+ sa(5a, +'a, !ai,a((8a *+(5a( !+hi*6a( !i,a 0+0+'a6a *ia(,a'a(8a

0+'ma(<aa, *a( 8a(5 lai((8a m+'5i!a(. Ba(8a! *ia(,a'a(8a m+(7a*i 6+(5h(i

*alam ,0h ma(sia. B+0+'a6a mi!'22'5a(ism+ m+(8+0a0!a( 6+(8a!i, *a( 8a(5

lai( ,+'li0a, *alam !+5ia,a( ma(sia 0+'ha'i9ha'i s+6+',i misal(8a 6+m0a,a( a(55'

!+7 825h', 6'2*!si 6+(i;illi( s+',a 6'2s+s96'2s+s 6+'la!a( 8a(5 0+'!ai,a(

*+(5a( 6+m0a(5a( lim0ah.

U7i s+(si=i,as 0a!,+'i m+'6a!a( ;a'a (,! m+(5+,ahi *a( m+(*a6a,!a(

6'2*! alam 8a(5 0+'62,+(si s+0a5ai 0aha( a(,i 0a!,+'i s+',a m+m6(8ai !+mam6a(

(,! m+(5ham0a, 6+',m0ha( a,a m+ma,i!a( 0a!,+'i 6a*a !2(s+(,'asi 8a(5

'+(*ah. U7i s+(si,i=i,as 0a!,+'i m+'6a!a( sa, m+,2*+ (,! m+(+(,!a( ,i(5!a,

!+'+(,a(a( 0a!,+'i ,+'ha*a6 /a, a(,i0a!,+'i *a( (,! m+(5+,ahi s+(8a>a m'(i

8a(5 m+mili!i a!,i=i,as a(,i0a!,+'i.

I.II T7a( P'a!,i!m



M+la!!a( 7i !+6+!aa( 0a!,+'i ,+'ha*a6 a(,i0i2,i!a (,! m+(5+,ahi 0a,as

!+6+!aa( a,a s+(si,i=i,as sa, 0a!,+'i "6+!a s+,+(5ah 6+!a a,a '+sis,+(& ,+'ha*a6

sa, a(,i0i2,i!a 8a(5 *i(8a,a!a( s+0a5ai !2(s+(,'asi ham0a, mi(imm "KHM& sa,

a(,i0i2,i!a.

I.III Ma(<aa, P'a!,i!m



S+,+lah m+la!!a( 6+';20aa( 7i !+6+!aa( 0a!,+'i ,+'ha*a6 a(,i0i2,i!a !i,a

*a6a, m+m0+*a!a( sa, a(,i0i2,i!a 8a(5 ,+6a, (,! *i5(a!a( s+0a5ai 6+(5ham0a,

6+'8m0ha( sa, 0a!,+'i 8a(5 ,+6a,. Mahasis>a *a6a, m+(5+,ahi !2(s+(,'asi

mi(imm sa, a(,i0i2,i!a *alam m+(5ham0a, 6+',m0ha( 0a!,+'i s+hi(55a ,i*a!

,+'7a*i !+l+0iha( a,a !+!'a(5a( !2(s+(,'asi. Mahasis>a *a6a, m+(5+,ahi

0a5aima(a m+!a(ism+ sa, a(,i0i2,i!a m+(5ham0a, 6+',m0ha( sa, 0a!,+'i.

BAB II



TINJAUAN PUSTAKA

A(,i0i2,i!a a*alah /a,9/a, !imia 8a(5 *ihasil!a( mi!'22'5a(ism+ hi*6 ,+',ama

<(5i *a( 0a!,+'i ,a(ah 8a(5 m+mili!i !hasia, m+ma,i!a( a,a m+(5ham0a, 6+',m0ha(

0a(8a! 0a!,+'i *a( 0+0+'a6a =i's 0+sa' s+*a(5!a( ,2!sisi,as(8a 0a5i ma(sia '+la,i< !+;il.

P+(55(aa( a(,i0i2,i! s+0a5ai 20a, 0a5i 6+(8a!i, 8a(5 *is+0a0!a( 2l+h i(<+!si

mi!'22'5a(ism+ ,+',ama 0a!,+'i sa(5a,lah am6h. Nam( 6+ma!aia( a(,i0i2,i! s+;a'a

,+'s m+(+'s *a6a, m+(8+0a0!a( '+sis,+((8a 0a!,+'i ,+'ha*a6 a(,i0i2,i!. T+'0+(,!(8a

'+sis,+(si mi!'20a ,+'ha*a6 a(,i0i2,i! *a6a, *ila!!a( *+(5a( ;a'a :

1. M+(;+5ah 6+ma!aia( a(,i0i2,i! (,! !ass 8a(5 ,i*a! m+m0,h!a(

#. M+(5h+(,i!a( 6+(55(aa( a(,i0i2,i! (,! i(<+!si 0iasa a,a s+0a5ai

20a, la' ). M+(55(a!a( a(,i0i2,i! 8a(5 ,+6a, *+(5a( *2sis 8a(5 ,+6a, a5a'

i(<+!si ;+6a, s+m0h

. M+(55(a!a( !2m0i(asi a(,i0i2,i! 8a(5 ,+lah ,+'0!,i !++<+!,i<a((8a

?. M+(55(a!a( a(,i0i2,i! 8a(5 lai( 0ila a*a ,a(*a9,a(*a 0ah>a mi!'20a

,+'s+0, '+sis,+( ,+'ha*a6 a(,i0i2,i! 8a(5 *i5(a!a(.

Tia6 s6+si+s mi!'20a m+mili!i ,i(55a, !+'+(,a(a( ,+',+(, ,+'ha*a6 a(,i0i2,i!. Ol+h

!a'+(a i, 6+'l *ila!!a( 7i !+6+!aa( "s+(si,i=i,as& mi!'20a ,+'ha*a6 a(,i0i2,i!. T+'*a6a,

0+0+'a6a m+,2*+ 8a(5 *a6a, *i5(a!a( (,! m+la!!a( 7i !+6+!aa( mi!'20a ,+'ha*a6

a(,i0i2,i!.

1. Metode dilusi

M+,2*+ *ilsi ,+'*i'i *a'i # ,+!(i! 6+(5+'7aa( 8ai, ,+!(i! *ilsi 6+'0+(iha(

;ai' *a( ,+!(i! *ilsi a5a'. M+,2*+ i(i 0+',7a( m+(+(,!a( a!,i<i,as a(,i0i2,i! s+;a'a

!a(,i,a,i<. M+,2*+ *ilsi *ila!!a( *+(5a( m+la',!a( a(,i0i2,i! !+*alam m+*ia a5a'

a,a !al* 8a(5 !+m*ia( *i,a(ami 0a!,+'i 8a(5 a!a( *i7i. S+,+lah *ii(!0asi a!a(

*i*a6a,!a( !2(s+(,'asi ,+'+(*ah a(,i0i2,i! 8a(5 *a6a, m+(5ham0a, 6+',m0ha(

0a!,+'i 8a(5 *is+0, *+(5a( !2(s+(,'asi Ham0a, Mi(imm "KHM& a,a Mi(imal

I(hi0i,2'8 2(;+(,'a,i2( "MI&.

a. Dilsi 6+'0+(iha( ;ai'

Dilsi 6+'0+(iha( ;ai' ,+'*i'i *a'i ma!'2*ilsi *a( mi!'2*ilsi. Pa*a

6'i(si6(8a ha(8a 0+'0+*a *alam =2lm+(8a sa7a. U(,! ma!'2*ilsi =2lm+ 8a(5

*i5(a!a( l+0ih *a'i 1 ml s+*a(5!a( mi!'2*ilsi =2lm+ 8a(5 *i5(a!a( $$? ml

sam6ai $1 ml. A(,imi!'20a 8a(5 *i5(a!a( *is+*ia!a( 6a*a 0+'0a5ai ma;am



6+(5+(;+'a( 0iasa(8a *alam sa,a( @5ml !2(s+(,'asi 0+'=a'iasi ,+'5a(,(5 7+(is *a(

si<a, a(,i0i2,i!

S+;a'a mm (,! 6+(+(,a( MI 6+(5+(;+'a( a(,imi!'20a *ila!!a(

6+('(a( !2(s+(,'asi s+,+(5ah(8a misal(8a mlai *a'i 1 % # 1 $? $#? @5ml&

!2(s+(,'asi ,+'+(*ah 8a(5 m+((7!!a( ham0a,a( 6+',m0ha( *+(5a( 7+las 0ai!

*iliha, s+;a'a =isal a,a ala, s+mi2,2ma,is *a( 2,2ma,is *is+0, *+(5a( !2(s+(,'asi

*a8a ham0a, mi(imm MI "Mi(imal I(hi0i,2'8 2(;+(,'a,i2(&.

0. Dilsi a5a'

Pa*a ,+!(i! *ilsi a5a' a(,i0i2,i! s+sai *+(5a( 6+(5+(;+'a( a!a(

*i,am0ah!a( !+*alam a5a' s+hi(55a a!a( m+m+'l!a( 6+'0+(iha( a5a' s+sai 7mlah

6+(5+;+'a( *i,am0ah sa, 6+'0+(iha( a5a' (,! !2(,'2l ,a(6a 6+(am0aha(

a(,i0i2,i!. K2(s+(,'asi ,+'+(*ah a(,i0i2,i! 8a(5 mam6 m+(5ham0a, 6+',m0ha(

0a!,+'i m+'6a!a( KHMMI a(,i0i2,i! 8a(5 *i 7i. Salah sa, !+l+0iha( m+,2*+

a5a' *ilsi (,! 6+(+(,a( MI Neisseria gonorrhoeae 8a(5 ,i*a! *a6a, ,m0h

6a*a ,+!(i! *ilsi 6+'0+(iha( ;ai'.

K+(,(5a( *a( !+'5ia( m+,2*+ *ilsi :

D+(5a( ,+!(i! *ilsi m+m(5!i(!a( 6+(+(,a( !ali,a,i< *a( !a(,i,a,i<

*ila!!a( 0+'s+ma9sama. KHM *a6a, m+m0a(, *alam 6+(+(,a( ,i(5!a, '+sis,+(si

*a( *a6a, m+(7a*i 6+,(7! 6+(55(aa( a(,i0i2,i!.

S+*a(5!a( !+'5ia( *a'i m+,2*+ i(i a*alah ,i*a! +<isi+( !a'+(a

6+(5+'7aa((8a 8a(5 'mi, m+m+'l!a( 0a(8a! ala, *a( 0aha( s+',a m+m+'l!a(

!+,+li,ia( *alam 6'2s+s 6+(5+'7aa((8a ,+'mas! 6+'sia6a( !2(s+(,'asi a(,imi!'20a

8a(5 0+'=a'iasi

#. M+,2*+ Di<si

Ba!,+'i *i,m0h!a( 6a*a m+*im l+m6+(5 a5a' !+m*ia( 0aha( a(,i0i2,i!

*i6a6a'!a( *+(5a( m+(+m6a,!a((8a *i a,as !+',as 0+'0+(,! ;a!'am "6a6+' *is!& *a(

*il+,a!a( *i a,as l+m6+(5 a5a' ,+'s+0,. M+*ia !+m*ia( *ii(!0asi!a( s+lama #

7am 6a*a sh )3$ . K+,aha(a( 0a!,+'i ,+'ha*a6 a(,i0i2,i! *iliha, 0+'*asa'!a(

*a+'ah ham0a, "/2(a hala& 8a(5

,+'0+(,! *i s+!+lili(5 6a6+' *is! a(,i0i2,i! ,+'s+0,. I(,+'6'+,asi ,+'ha*a6

/2(a ham0a, :

a. Da+'ah ham0a, *+(5a( *iam+,+' l+0ih *a'i )$ mm m+((7!a( 0ah>a 0a!,+'i ,+'s+0,

peka ,+'ha*a6 a(,i0i2,i!

0. Da+'ah ham0a, *+(5a( *iam+,+' a(,a'a #$ C )$ mm m+((7!a( 0ah>a 0a!,+'i

,+'s+0, agak resisten ,+'ha*a6 a(,i0i2,i!

;. Da+'ah ham0a, *+(5a( *iam+,+' !'a(5 *a'i #$ mm m+((7!a( 0ah>a 0a!,+'i

,+'s+0, resisten ,+'ha*a6 a(,i0i2,i!.



BAB III

METODOLOGI

III. I Ala, *a( Baha(

• Ala,

o Pi6+,96i6+, s,+'il

o Ta0(59,a0(5 s,+'il

o a>a( 6+,'i s,+'ilo Pi(s+,



o K+',as ;a!'am a,a 6+;a*a(5 l25am s,+'il

o OHP 6+(

• Baha(

o La',a( 6+(5+(;+' a(,i0i2,i!a T+','asi!li( Hl "1$$@5mL *a( mlai

1$$$@5mL 0+',',9,', *i0a5i # sam6ai 1$ 6+(5+(;+'a(&o Ss6+(si 0ia!a( Staphyloccocus aureus *a( Eschericia coli *alam

!al* 6+6,2( "# 7am #?T&

o Aa*+s,

o M+*ia !al* 6+6,2(+ s,+'il

o M+*ia a5a' 0+'sh F%

o M+*ia a5a' ;ai' 0+'sh %

III. II a'a K+'7a

A. a'a P+(i6isa( S+'i Kal* P+6,2( "Serial Broth Dilution Method &

1. M+(8ia6!a( 6+(i6isa( 0ia!a( 0a!,+'i 1:1$$$

a. Sia6!a( ,a0(5 s,+'il *a( 0+'i (2m2' 1 sam6ai *+(5a(

0. K+ *alam ,a0(5 (2.1 *a( # masi(59masi(5 *imas!!a( #3 mL !al*

6+6,2( *a( !+ *alam ,a0(5 (2.) *a( masi(59masi(5 - mL.

;. K+ *alam ,a0(5 (2.1 *imas!!a( $) mL ss6+(si 0ia!a(

Staphyloccocus aureus "# 7am #?T& !+m*ia( h2m25+(!a(. Ma!a

6a*a ,a0(5 (2.1 ,+'*a6a, 6+(5+(;+'a( 0a!,+'i 1:1$

*. Am0il $) mL *a'i (2.1 lal *imas!!a( !+ *alam ,a0(5 (2.#. Ma!a

6a*a ,a0(5 (2.# ,+'*a6a, 6+(5+(;+'a( 0a!,+'i 1:1$$

+. Da'i ,a0(5 (2.# 6i(*ah!a( masi(59masi(5 1 mL !+ *alam ,a0(5 (2.)

*a( . Ma!a 6a*a ,a0(5 (2.) *a( ,+'*a6a, 6+(5+(;+'a( 0a!,+'i 1:1$$$

#. Sia6!a( 1$ ,a0(5 s,+'il *alam 'a! 0+'i (2m2' 1 sam6ai *+(5a( 1$

). K+ *alam ,a0(5 (2.# sam6ai *+(5a( 1$ masi(59masi(5 *imas!!a( $? mL

m+*ia !al* 6+6,2(

. K+ *alam ,a0(5 (2.1 *a( # masi(59masi(5 *imas!!a( $? mL +(;+'a(

a(,i0i2,i!a "T+,'asi!li( Hl& *+(5a( !2(s+(,'asi ,+',+(, "1$$@5mL&

!+m*ia( h2m25+(!a(?. Pi(*ah!a( s+0a(8a! $? mL *a'i ,a0(5 (2.# !+ ,a0(5 (2.) h2m25+(!a(

lal 6i(*ah!a( $? mL *a'i ,a0(5 (2.) !+ ,a0(5 (2. h2m25+(!a( *a(

s+,+'s(8a sam6ai *+(5a( ,a0(5 (2.1$

. Mas!!a( !+ *alam ,a0(59,a0(5 1 sam6ai *+(5a( 1$ 6+(i6isa( 0a!,+'i

1:1$$$ masi(59masi(5 s+0a(8a! 1? mL !+m*ia( h2m25+(!a(

7. I(!0asi *alam i(!0a,2' )?9)3 s+lama 1%9# 7am *a( *i6ilih 6a*a

!2(s+(,'asi a(,i0i2,i! ,+'+(*ah ma(a!ah ,+'*a6a, 6+(5ham0a,a( 8a(5

s+m6'(a ,+'ha*a6 6+',m0ha( 0a!,+'i. K2(s+(,'asi a(,i0i2,i!a ,+'+(*ahi(ilah 8a(5 *is+0, s+0a5ai “0a,as !+6+!aa( 0a!,+'i” ,+'ha*a6 a(,i0i2,i!a



,+'s+0,. Ba,as !+6+!aa( 0a!,+'i mm(8a *is+0, *+(5a( konsentrasi

hambat minimum (KHM ! minimal inhibitory concentration (M"#

B. a'a Di<si A5a' "$he %gar Di&&usion Method ! Medicated 'aper Disc Method &

M+(55(a!a( 0a!,+'i Staphyloccocus aureus

1. Pi6+,!a( $1 mL 0ia!a( Staphyloccocus aureus !+ *alam ;a>a( 6+,'i

s,+'il !+m*ia( ,a(5!a( a5a' ;ai' 0+'sh % h2m25+(!a(

0ia'!a( m+ma*a,. S+,+lah m+ma*a, sim6a( *alam i(!0a,2' 0+'sh

)3 *+(5a( 62sisi ;a>a( *i0ali! sam6ai ,i,i! a6 ai' 8a(5 0+'a*a *i

6+'m!aa( a5a' hila(5. Ba5ia( *asa' ;a>a( *i0a5i m+(7a*i ) 0a5ia(

*+(5a( m+(55(a!a( OHP 6+(. Ta(*ai (,! *2sis '+(*ah "R&

m+(+(5ah "M& *a( ,i(55i "T&

#. D+(5a( m+(55(a!a( 6i(s+, s,+'il am0il !+',as ;a!'am a,a 6+;a*a(5l25am s,+'il *a( 7+(h!a( *+(5a( ;ai'a( a(,i0i2,i!a ,+',+(, *a(

l+,a!!a( *i 6+'m!aa( a5a' 8a(5 ,+lah m+(5a(*(5 ss6+(si 0a!,+'i

s+sai *+(5a( !2(s+(,'asi 8a(5 a!a( *i7i

). I(!0asi!a( *alam i(!0a,2' 0+'sh )3 s+lama 1%9# 7am

. Ama,i *a( !' *iam+,+' *a+'ah ham0a, 8a(5 *ihasil!a(

M+(55(a!a( 0a!,+'i Eschericia coli

1. Pi6+,!a( $1 mL 0ia!a( Eschericia coli !+ *alam ;a>a( 6+,'i s,+'il

!+m*ia( ,a(5!a( a5a' ;ai' 0+'sh % h2m25+(!a( 0ia'!a(

m+ma*a,. S+,+lah m+ma*a, sim6a( *alam i(!0a,2' 0+'sh )3

*+(5a( 62sisi ;a>a( *i0ali! sam6ai ,i,i! a6 ai' 8a(5 0+'a*a *i

6+'m!aa( a5a' hila(5. Ba5ia( *asa' ;a>a( *i0a5i m+(7a*i ) 0a5ia(

*+(5a( m+(55(a!a( OHP 6+(. Ta(*ai (,! *2sis '+(*ah "R&

m+(+(5ah "M& *a( ,i(55i "T&

#. D+(5a( m+(55(a!a( 6i(s+, s,+'il am0il !+',as ;a!'am a,a 6+;a*a(5

l25am s,+'il *a( 7+(h!a( *+(5a( ;ai'a( a(,i0i2,i!a ,+',+(, *a(

l+,a!!a( *i 6+'m!aa( a5a' 8a(5 ,+lah m+(5a(*(5 ss6+(si 0a!,+'i

s+sai *+(5a( !2(s+(,'asi 8a(5 a!a( *i7i

). I(!0asi!a( *alam i(!0a,2' 0+'sh )3 s+lama 1%9# 7am

. Ama,i *a( !' *iam+,+' *a+'ah ham0a, 8a(5 *ihasil!a(



BAB IV

HASIL PENGAMATAN DAN PEMBAHASAN

IV.I Hasil P+(5ama,a(

1. Cara Dilusi

Nama A(,i0i2,i! : Kl2'am<+(i!2lBa!,+'i 7i : Staphyloccocus aureus

N2. Ta0(5 1 # ) ? 3 % - 1$

D2sis

A(,i0i2,i!

"@5ml&

1$$ ?$ #? 1#? #? )1#? 1?) $3%# $)-1 $1-

P+',m0ha(

Ba!,+'i9 9 9 9 9 9

B+'*asa'!a( hasil 6+(5ama,a( ,+'s+0, *ia,as ma!a !2(s+(,'asi ham0a, mi(imm

"KHM& a(,i0i2,i! Kl2'am<+(i!2l ,+'ha*a6 0a!,+'i Staphyloccocus aureus a*alah

)1#? @5mL

2. Cara Difusi

a. Nama a(,i0i2,i! : Kl2'am<+(i!2l

Ba!,+'i 7i : Staphyloccocus aureus

Gam0a' D2sis A(,i0i2,i! "@5mL&

R " 1$ @5mL& M "?$ @5mL& T "1$$ @5mL&



DDH 9 DDH 11 mm DDH 1- mm

B+'*asa'!a( hasil 6+';20aa( ,+'s+0' *ia,as ma!a 0a!,+'i 7i 0+'si<a, s+,+(5ah 6+!a

,+'ha*a6 a(,i0i2,i!.

0. Nama a(,i0i2,i! : Kl2'am<+(i!2l

Ba!,+'i 7i : Eschericia coli

Gam0a' D2sis A(,i0i2,i! "@5mL&

R " 1$ @5mL& M "?$ @5mL& T "1$$ @5mL&

DDH 9 DDH 9 DDH % mm

B+'*asa'!a( hasil 6+';20aa( *ia,as ma!a 0a!,+'i 7i 0+'si<a, s+*i!i, 6+!a

,+'ha*a6 a(,i0i2,i!.

IV.II P+m0ahasa(

IV.II.I P+m0ahasa( a'a Dilsi

• Ma!s* *a'i 6+';20aa( 7i s+(si,i=i,as 0a!,+'i ,+'ha*a6 a(,i0i2,i!

a*alah (,! m+(5+,ahi 6+(5a'h sa, a(,i0i2,i! ,+'ha*a6

6+',m0ha( 0a!,+'i *ima(a ,i(5!a, s+(si,i=i,as ,+'s+0, *a6a,

*i*+s!'i6si!a( s+0a5ai sa(5a, 6+!a 6+!a s+,+(5ah 6+!a s+*i!i, 6+!a

*a( '+sis,+(.

• Dalam 6+';20aa( i(i ,+'*a6a, # m+,2*+ (,! m+(57i s+(si,i=i,as

0a!,+'i ,+'ha*a6 a(,i0i2,i! 8ai, m+,2*+ *ilsi *a( m+,2*+ *i<si

a5a'.

ssss

ssss



• M+,2*+ *ilsi "6+(i6isa( s+'i !al* 6+6,2(& *ila!!a( *+(5a(

m+m0a, s+'i 6+(5+(;+'a( a(,i0i2,i! 8a(5 masi(59masi(5 *i0+'i

m+*ia !al* 6+6,2( *a( *ii(2!lasi *+(5a( ss6+(si 0a!,+'i.

•

Sa, 0a!,+'i s+(si,i= ,+'ha*a6 a(,i0i2,i! *a6a, *iliha, *a'i !+!+'ha(m+*ia a6a0ila ,+'7a*i !+!+'ha( m+*ia 0+'a',i 62si,i< ,+'7a*i sa,

6+',m0ha( 0a!,+'i s+*a(5!a( a6a0ila ,i*a! ,+'7a*i !+!+'ha(

0+'a',i ,i*a! ,+'7a*i 6+',m0ha( 0a!,+'i "(+5a,i<&.

• Pa*a m+,2*+ *ilsi *a6a, *i6+'2l+h *a,a !a(,i,a,i< *a'i !2(s+(,'asi

ham0a, mi(imm "KHM&. K2(s+(,'asi ham0a, mi(imm *a6a,

*i,+(,!a( *a'i *2sis a(,i0i2,i! ,+'!+;il 8a(5 masih *a6a,

m+(5ham0a, 6+',m0ha( 0a!,+'i. Bila 6a*a !2(s+(,'asi ,+',+(,,i*a! ,+'7a*i !+!+'ha( m+*ia lal !2(s+(,'asi 8a(5 l+0ih !+;il

s+la(7,(8a ,+'*a6a, !+!+'ha( 0+'a',i KHM9(8a a*alah *2sis

a(,i0i2,i! 8a(5 ,i*a! ,+'7a*i !+!+'ha( m+*ia.

• Dalam 6+';20aa( i(i a(,i0i2,i! 8a(5 a!a( *i5(a!a( a*alah

!l2'am<+(i!2l *+(5a( *2sis a>al 1$$ @5ml 8a(5 *i+(;+'!a( 1:#

s+0a(8a! 1$ !ali. Ka'+(a *a'i ,a0(5 191$ *2sis a(,i0i2,i! 8a(5

*i0+'i!a( s+ma!i( s+*i!i, "+(;+'& s+ha's(8a s+ma!i( 0+',am0ah

!+!+'ha((8a. Hal i(i !a'+(a s+ma!i( !+;il *2sis a(,i0i2,i! 8a(5

*i0+'i!a( !+!a,a((8a s+ma!i( !+;il (,! m+(5ham0a,

6+',m0ha( 0a!,+'i. S+*a(5!a( 0a!,+'i 8a(5 a!a( *i5(a!a( a*alah

Staphylococcus aureus.

• B+'*asa'!a( hasil 6+(5ama,a( *a'i ,a0(5 8a(5 *i0+'i *2sis

!l2'am<+(i!2l s+0a(8a! 1$$ @5ml C )1#? @5ml ,i*a! ,+'7a*i

!+!+'ha( s+*a(5!a( mlai *a'i ,a0(5 *+(5a( *2sis !l2'am<+(i!2l

1?) C $1- @5ml ,+'7a*i !+!+'ha(. D+(5a( *+mi!ia( (ilai KHM9

(8a a*alah )1#? @5ml. M+(', Ta0+l 11.1 6a*a P+((,(

P'a!,i!m Mi!'20i2l25i (,! !l2'am<+(i!2l *+(5a( KHM )1#?

@5ml ,+'mas! *alam !a,+52'i 0a!,+'i 8a(5 sa(5a, 6+!a. D+(5a(

*+mi!ia( 0a!,+'i Staphylococcus aureus sa(5a, 6+!a ,+'ha*a6

a(,i0i2,i! !l2'am<+(i!2l.



IV.II.II P+m0ahasa( a'a Di<si

• Pa*a 6+';20aa( m+,2*+ *i<si !ali i(i a5a' 8a(5 6+',ama *i5(a!a(

a*alah Straphylococcus aureus s+0a5ai 0a!,+'i 7i *a( Kl2'am<+(i!2l

s+0a5ai a(,i0i2,i!a.• Pa*a m+*ia a5a' l+m6+(5 *i0a5i m+(7a*i ,i5a 0a5ia(8ai, 0a5ia(

'+(*ah 0a5ia( m+(+(5ah *a( 0a5ia( ,i(55i.

• Pa*a *2sis a(,i0i2,i!a 0a5ia( '+(*ah "1$ m5ml& *i*a6a,!a(

*iam+,+' *a+'ah ham0a, $ mm !+m*ia( 6a*a *2sis a(,i0i2,i!a 0a5ia(

m+(+(5ah "?$ m5ml& *i*a6a,!a( *iam+,+' *a+'ah ham0a, 11 mm

*a( 6a*a *2sis a(,i0i2,i!a 0a5ia( ,i(55i "1$$ m5ml& *i*a6a,!a(

*iam+,+' *a+'ah ham0a, 1- mm.• Hasil 6+';20aa( i(i m+((7!!a( 0ah>a 0a!,+'i Staphylococcus

aureus 0+'si<a, s+,+(5ah 6+!a ,+'ha*a6 a(,i0i2,i!a !l2'am<a(i!2l. Hal

i(i *a6a, *iliha, *+(5a( ,i*a! a*a(8a *iam+,+' *a+'ah ham0a, 8a(5

*ihasil!a( *i s+!i,a' !+',as ;a!'am *+(5a( !2(s+(,'asi a(,i0i2,i!a

0a5ia( '+(*ah ,+,a6i a*a 6a*a !2(s+(,'asi 8a(5 m+(+(5ah *a( ,i(55i.

• Pa*a 6+';20aa( m+,2*+ *i<si a5a' 8a(5 !+*a m+(55(a!a(

Escherichia coli s+0a5ai 0a!,+'i 7i *a( !l2'am<+(i!2l s+0a5aia(,i0i2,i!a.

• M+*ia a5a' l+m6+(5 75a *i0a5i m+(7a*i ,i5a 0a5ia( 8ai, '+(*ah

m+(+(5ah *a( ,i(55i.

• Pa*a *2sis a(,i0i2,i!a '+(*ah "1$ m5ml& *i*a6a,!a( *iam+,+' *a+'ah

ham0a, $ mm !+m*ia( 6a*a *2sis a(,i0i2,i!a m+(+(5ah "?$

m5ml& *i*a6a,!a( *iam+,+' *a+'ah ham0a, $ mm *a( 6a*a *2sis

a(,i0i2,i!a ,i(55i "1$$ m5ml& *i*a6a,!a( *iam+,+' *a+'ah ham0a, %mm.

• Hasil 6+';20aa( i(i m+((7!!a( 0ah>a 0a!,+'i 7i Escherichia coli

0+'si<a, s+*i!i, 6+!a ,+'ha*a6 a(,i0i2,i!a !l2'am<+(i!2l. Hal i(i *a6a,

*iliha, *+(5a( ,i*a! a*a(8a *iam+,+' *a+'ah ham0a, 6a*a !+',as

;a!'am *+(5a( !2(s+(,'asi la',a( 6+(5+(;+'a( a(,i0i2,i! 8a(5 '+(*ah

*a( m+(+(5ah ,+,a6i ,+'*a6a, 6a*a !2(s+(,'asi 8a(5 ,i(55i.



BAB V

KESIMPULAN

B+'*asa'!a( hasil 6+(5ama,a( 7i s+(si,i=i,as 0a!,+'i ,+'ha*a6 a(,i0i2,i!a

*+(5 m+,2*+ *ilsi *a6a, *isim6l!a( 0ah>a 0a!,+'i Staphylococcus aureus *+(5a(

*2sis 1?) C $1- @5ml sa(5a, 6+!a ,+'ha*a6 a(,i0i2,i! !l2'am<+(i!2l !a'+(a

,+'7a*i !+!+'ha( 6a*a ,a0(5 *a( (ilai KHM9(8a a*alah )1#? @5ml 8a(5

m+(', Ta0+l 11.1 6a*a P+((,( P'a!,i!m Mi!'20i2l25i (,! !l2'am<+(i!2l

*+(5a( KHM )1#? @5ml ,+'mas! *alam !a,+52'i 0a!,+'i 8a(5 sa(5a, 6+!a.

S+*a(5!a( 0+*a*a'!a( hasil 6+(5ama,a( 7i s+(si,i=i,as 0a!,+'i ,+'ha*a6

a(,i0i2,i!a *+(5 m+,2*+ *i<si 0ah>a 6a*a 0a!,+'i Staphylococcus aureus 0+'si<a,

s+,+(5ah 6+!a ,+'ha*a6 a(,i0i2,i!a !l2'am<a(i!2l. Hal i(i *a6a, *iliha, *+(5a( ,i*a!

a*a(8a *iam+,+' *a+'ah ham0a, 8a(5 *ihasil!a( *i s+!i,a' !+',as ;a!'am *+(5a(

!2(s+(,'asi a(,i0i2,i!a 0a5ia( '+(*ah ,+,a6i a*a 6a*a !2(s+(,'asi 8a(5 m+(+(5ah

*a( ,i(55i. S+*a(5!a( 6a*a 0a!,+'i 0a!,+'i 7i Escherichia coli 0+'si<a, s+*i!i, 6+!a

,+'ha*a6 a(,i0i2,i!a !l2'am<+(i!2l. Hal i(i *a6a, *iliha, *+(5a( ,i*a! a*a(8a *iam+,+'

*a+'ah ham0a, 6a*a !+',as ;a!'am *+(5a( !2(s+(,'asi la',a( 6+(5+(;+'a( a(,i0i2,i!

8a(5 '+(*ah *a( m+(+(5ah ,+,a6i ,+'*a6a, 6a*a !2(s+(,'asi 8a(5 ,i(55i



BAB VI

DA4TAR PUSTAKA

9 Tim P+(8s( La02'a,2'im Mi!'20i2l25i 4a!l,as 4a'masi U(i=+'si,as

Pa(;asila. #$1#.P+((,( P'a!,i!m Mi!'20i2l25i. Ja!a',a: 44UP.

9 al82 L*. #$$%. T+!(i! *a( M+,2*+ Dasa' Dalam Mi!'20i2l25i. Mala(5: UMM

P'+ss.

9 D>i*72s+6,'2 D. 1--%. Dasa'9Dasa' Mi!'20i2l25i. D7am0a,a(. Ja!a',a



Da<,a' 6+m0a5ia( ,5as:

Ba0 1: Di(*a P+'ma,asa'i

Ba0 #: Eli/a0+,h a'2li(+ S

Ba0 ): Diah K'is(a

Ba0 : Dhimas Khalis

Ba0 ?: Dhimas Khalis Diah K'is(a Di(*a P+'ma,asa'i Eli/a0+,h a'2li(+ S

bab i uji sensivitas bakteri

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